Journal: Journal of Medical Biochemistry

Article Title: Correlation and clinical significance of placental tissue selectin (E), angiotensin II and its receptors, and oxidized lipid levels in patients with preeclampsia

doi: 10.5937/jomb0-51303

Figure Lengend Snippet: Comparison of relevant data between healthy group and case group (x̄±S).

Article Snippet: Remove the placental tissue from the refrigerator and thaw at room temperature. (1) Selectin (E) Immunohistochemical SP method (Beijing Zhong shan Biotechnology Co., Ltd.) was used, and the placenta tissue was subjected to paraffin sectioning and immunohistochemical staining in strict accordance with the instructions.

Techniques: Comparison

Journal: Journal of Medical Biochemistry

Article Title: Correlation and clinical significance of placental tissue selectin (E), angiotensin II and its receptors, and oxidized lipid levels in patients with preeclampsia

doi: 10.5937/jomb0-51303

Figure Lengend Snippet: Comparison of the expression levels of selectin (E), AngII, ATRs, MDA, 8-iso-PGF2a and the levels of SBP and DBP in the two groups (***P<0.001).

Article Snippet: Remove the placental tissue from the refrigerator and thaw at room temperature. (1) Selectin (E) Immunohistochemical SP method (Beijing Zhong shan Biotechnology Co., Ltd.) was used, and the placenta tissue was subjected to paraffin sectioning and immunohistochemical staining in strict accordance with the instructions.

Techniques: Comparison, Expressing

Journal: Journal of Medical Biochemistry

Article Title: Correlation and clinical significance of placental tissue selectin (E), angiotensin II and its receptors, and oxidized lipid levels in patients with preeclampsia

doi: 10.5937/jomb0-51303

Figure Lengend Snippet: Correlation analysis between placental tissue selectin (E), AngII, ATRs, MDA, 8-iso-PGF2a expression and SBP and DBP levels.

Article Snippet: Remove the placental tissue from the refrigerator and thaw at room temperature. (1) Selectin (E) Immunohistochemical SP method (Beijing Zhong shan Biotechnology Co., Ltd.) was used, and the placenta tissue was subjected to paraffin sectioning and immunohistochemical staining in strict accordance with the instructions.

Techniques: Expressing

Journal: Journal of Medical Biochemistry

Article Title: Correlation and clinical significance of placental tissue selectin (E), angiotensin II and its receptors, and oxidized lipid levels in patients with preeclampsia

doi: 10.5937/jomb0-51303

Figure Lengend Snippet: Correlation analysis between placental tissue selectin (E), AngII, ATRs, MDA, 8-iso-PGF2a expression and SBP and DBP levels.

Article Snippet: Remove the placental tissue from the refrigerator and thaw at room temperature. (1) Selectin (E) Immunohistochemical SP method (Beijing Zhong shan Biotechnology Co., Ltd.) was used, and the placenta tissue was subjected to paraffin sectioning and immunohistochemical staining in strict accordance with the instructions.

Techniques: Expressing

Journal: Journal of Medical Biochemistry

Article Title: Correlation and clinical significance of placental tissue selectin (E), angiotensin II and its receptors, and oxidized lipid levels in patients with preeclampsia

doi: 10.5937/jomb0-51303

Figure Lengend Snippet: Analysis of the value of placental tissue selectin (E), AngII, ATRs, MDA, and 8-iso-PGF2a expression alone and jointly in predicting the risk of PE.

Article Snippet: Remove the placental tissue from the refrigerator and thaw at room temperature. (1) Selectin (E) Immunohistochemical SP method (Beijing Zhong shan Biotechnology Co., Ltd.) was used, and the placenta tissue was subjected to paraffin sectioning and immunohistochemical staining in strict accordance with the instructions.

Techniques: Expressing

Journal: Lipids in health and disease

Article Title: Knockdown of RASD1 improves MASLD progression by inhibiting the PI3K/AKT/mTOR pathway.

doi: 10.1186/s12944-024-02419-z

Figure Lengend Snippet: Fig. 1 Hepatic RASD1 was upregulated in patients with MASLD. (A) qPCR analysis showing the RASD1 mRNA expression levels in liver tissues from both groups (n = 15 each). (B) WB analysis of RASD1 protein levels and the corresponding quantitative analysis of liver tissues from the Normal and MASLD groups (n = 6). (C) Representative HE and IHC staining of liver tissues from the Normal and MASLD groups (scale bar, 50 μm), on the right of which the IHC staining data is quantified and shown (n = 3). (D) Representative IF staining of liver tissues from the Normal and MAFLD groups (scale bar: 20 μm). **P < 0.01, ****P < 0.0001, comparing to those data of the Normal group. All data presented in the figure are means ± SDs

Article Snippet: For immunofluorescent (IF) staining, the IHC experimental procedure was followed until the primary antibody (RASD1, A05991-1, BOSTER, Wuhan, China) was incubated.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Lipids in health and disease

Article Title: Knockdown of RASD1 improves MASLD progression by inhibiting the PI3K/AKT/mTOR pathway.

doi: 10.1186/s12944-024-02419-z

Figure Lengend Snippet: Fig. 6 RASD1 knockdown improved MASLD development in vivo. (A) Schematic diagram of the animal study. (B) Hepatic Rasd1 mRNA levels in mice from four groups (n = 5–6). (C) Hepatic RASD1 protein levels and the corresponding quantitative analysis of mice from four groups (n = 3). (D) Representa tive images showing the gross look of the liver as well as staining of HE, IHC and ORO under microscope (scale bar: 50 μm) of mice from four groups. The quantitative analysis results of IHC and ORO staining are shown on the right (n = 3). (E) Body weights of the mice from four groups (n = 5). (F, G) results of the GTT and ITT detected in different time in the mice assigned to each group (n = 5–6). (H-K) AUC and AOC for the GTT and ITT results (n = 5–6). (L-P) Serum levels of liver function assays and insulin in the mice from four groups (n = 5–6). (Q) TG levels in the mice liver tissues from four groups (n = 5–6). With the NCD group as control comparison, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; With the AAV-shNC/HFD group as control comparison, #P < 0.05, ##P < 0.01, ####P < 0.0001, which are compared to the group. All data presented in the figure are means ± SDs

Article Snippet: For immunofluorescent (IF) staining, the IHC experimental procedure was followed until the primary antibody (RASD1, A05991-1, BOSTER, Wuhan, China) was incubated.

Techniques: Knockdown, In Vivo, Staining, Microscopy, Control, Comparison

Journal: Frontiers in Immunology

Article Title: The immunological and prognostic significance of the diabetes mellitus-related gene WFS1 in endometrial cancer

doi: 10.3389/fimmu.2024.1464421

Figure Lengend Snippet: Differential expression of WFS1 in tumors and survival analysis. (A) Expression of WFS1 in pan-cancer. (* p < 0.05, ** p < 0.01, and *** p < 0.001). (B) The mRNA expression levels of WFS1 in EC and normal. (C) The mRNA expression levels of WFS1 in EC and normal groups (GSE17025, GSE63678 and GSE115810 datasets). (D) Expression levels of WFS1 are used to assist in the diagnosis of EC. (E) The mRNA expression levels of WFS1 in EC and matched-adjacent normal. (F) The mRNA expression levels of WFS1 in different grades EC and normal. (G) KM curves showed shorter OS in the WFS1 low expression group. (H, I) The EC group and the paracancerous tissues were subjected to comparative analysis through IHC scoring. (H) indicates an overall difference. (I) indicates compared differences. (J) IHC of WFS1 in normal tissues (upper: 100um, lower: 50um). (K) IHC of WFS1 in tumor tissues (upper: 100um; lower: 50um).

Article Snippet: IHC procedures were performed in strict accordance with the instructions of the WFS1 antibody (IHCeasy WFS1 Ready-To-Use IHC Kit, ProteinTech, Cat No: KHC0048).

Techniques: Quantitative Proteomics, Expressing, Biomarker Discovery